Structure of amide
In the amide molecule, the nitrogen atom is sp2 hybridized, and the p-orbital of the lone pair electron and the carbonyl group form a P-Π conjugate. As a result of the conjugation, not only the electron cloud density and bond length in the amide molecule tend to be averaged, but also the rotation of the CN single bond is blocked. . The planar configuration of the amide greatly affects the physical and chemical properties of the amide and the spatial structure of the protein.