Biological plasticization step
(1) Fixation: It is mainly fixed with formalin.
(2) Dehydration and degreasing: use acetone as a dehydrating agent, degreasing agent and intermediary solvent under vacuum.
(3) Forced infiltration: Under vacuum conditions, replace the acetone in the tissue with polymer liquids such as silicone rubber and epoxy resin.
(4) Polymerization: Use gas, light or heating to polymerize the polymers in the specimen.