Advantages of spin labeling
① Sensitive to the polarity of the solvent, so it is possible to explore the hydrophobicity or hydrophilicity of the surrounding environment of the marker;
②It is extremely sensitive to the rate of molecular rotation, so it can measure the degree of activity allowed in the environment of the marker, especially the change in the conformation of biomolecules caused by a certain biochemical process;
③The EPR spectrum is relatively simple and easy to analyze. The three-peak spectrum caused by 14N can provide a lot of valuable information;
④ There is no interference signal from diamagnetic environment.