Time-of-flight mass spectrometry

The surface-enhanced laser desorption ionization time-of-flight mass spectrometry technology was invented by Nobel Prize winner Tanaka in 2002, and it has attracted great attention from the academic community just after it was produced. SELDI technology is an ideal technology platform in proteomics research. Its full name is surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-tof). The method is mainly as follows: under normal circumstances, the sample is directly dripped onto a specially modified chip after a simple pretreatment, which can compare the difference protein between the two samples and obtain the protein overview of the sample. Therefore, it has significant advantages in application. The samples analyzed by SELDI technology do not need to be pre-purified by liquid chromatography or gas chromatography, so it can be used to analyze complex biological samples. SELDI technology can analyze hydrophobic proteins, proteins with too high or too low PI, and proteins with low molecular weight (< 25 000). It can also find many low-concentration proteins that are masked in untreated samples, increasing the discovery of biomarkers Opportunity. SELDI technology requires only a small amount of samples, results can be obtained in a short time, and the test is reproducible, suitable for clinical diagnosis and large-scale screening of disease-related biomarkers, especially it can directly detect untreated urine , Blood, cerebrospinal fluid, joint cavity synovial fluid, bronchial effluent, cell lysate and various secretions, etc., so that the molecular weight, PI, glycosylation site, phosphorylation site and other parameters of the target protein in the sample can be detected .